Multiple Generational Bovine Embryo Cloning
نویسندگان
چکیده
منابع مشابه
Oocyte genome cloning used in biparental bovine embryo reconstruction.
Oocyte genome cloning is a method by which haploid maternal embryos are obtained in such a way that parthenogenetic haploid blastomeres from these embryos can be considered as a clone of the original gamete. Our objective was to generate oocyte genome replicates and use them to reconstruct biparental embryos by fusion with haploid male hemizygotes. Furthermore, we generated biparental homogeneo...
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On a worldwide basis, more than 750,000 embryos are produced annually from superovulated donors and more than 450,000 embryos are produced using in vitro techniques. Superovulation and embryo collection are done as frequently as every 30 days. Cryopreservation and direct transfer of frozen-thawed embryos results in pregnancy rates near that of fresh embryos. Since the zona pellucida-intact in v...
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Bovine oocytes were matured, fertilized, and cultured FCM 199 with serum and co-culture) in vitro (IVMFC) with addition, during different phases of the procedure, of antioxidants: superoxide dismutase (SOD) and reduced glutathione (GSH). The addition of SOD (1,500 or 3,000 IU/ml) did not improve proportions of oocytes undergoing cleavage or the development of embryos to morula and blastocyst st...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1993
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod48.4.715